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in vitro alternative method test

china is a big country in the production and consumption of skin care products. in recent years, with the improvement of the national income level and the upgrading of consumption concepts, the market size of skin care products has expanded significantly, and the competition in the industry has become increasingly fierce. at a time when new brands, new effects, and new ingredients are constantly emerging, consumers pay special attention to the safety of product ingredients and the authenticity of their effects. the state food and drug administration's inspection and supervision of endless skin care products on the market is also increasing year by year.

with the increasingly strict requirements of the eu cosmetic regulations on the prohibition of laboratory animals, banned and restricted substances and microorganisms in cosmetics, and the continuous promotion of the 3r (replace, optimize, and reduce) principles of laboratory animal ethics, the reduction and cancellation of cosmetic animal testing for safety evaluation has been more and more countries recognized.

noa provides cosmetics safety and efficacy evaluation services for enterprises based on new technologies such as cell culture, tissue engineering and systems biology with professional testing capabilities and a first-class in vitro efficacy testing platform. at the same time, noa can provide one-stop targeted solutions according to the specific needs of enterprises, helping enterprises to develop and innovate.

business content

1. in vitro efficacy testing

project name	method
moisture	detection of aquaporin-3 (a qp3) expression in vivo
	hyaluronic acid content in the body (cells)
	3d epidermis model moisturizing test
crease resistant	collagen protein enzyme inhibition (biochemical)
	efficacy detection of mmp i protein inhibition
	detection of free radical scavenging capacity such as dpph (biochemical)
	mitochondrial membrane potential detection (cell)
	detection of reactive oxygen species (ros) clearance capacity in keratinocytes
	fibroblast type i collagen promotes efficacy detection
compact	elastase inhibition (biochemical)
	detection of free radical scavenging capacity such as dpph (biochemical)
	detection of reactive oxygen species (ros) clearance capacity in keratinocytes
	elastin generation (cell)
leisurely	hyaluronidase inhibition (biochemical)
	efficacy of tnf α
	inflammatory factor inhibition (double factor) (cell)
	in vitro degranulation detection of mast cells
	tdetection of trpv1 receptor inhibition efficacy
temperate no stimulation	keratin cytotoxicity assays
	detection of het-cam eye irritation in chick embryo
	rabbit corneal epithelial cells (sirc) eye irritation detection
	eye irritation test in the 3d corneal model
	3d epidermal cell model
oil-control	5- α reductase inhibition (biochemical)
oil-control	detection of the oil-control efficacy of s z95 sebaceous gland cells
anti-dangdruff	malassezia species, the antibacterial rate
whitening	detection of the whitening efficacy of in vitro recombinant 3d melanin model
	b 16 melanocytic internal melanin inhibition assay
	detection of tyrosinase activity inhibition efficacy (biochemical)

service process

q&a

what is the chicken embryo villous allanic membrane? why can using chicken embryo villous allanic membrane predict eye irritation?

chicken embryo villus allantoic membrane test is an early adopted method of ocular irritation in vitro evaluation. the villus allantoic membrane (cam) is a respiratory membrane, surrounded by the chicken embryo, and its structure is similar to the eye conjunctiva.

this experiment uses the characteristics of the complete, clear and transparent, to observe the toxic effect index (such as bleeding, coagulation, and vascular melting), which reflect the morphology, color, and permeability of the protein degeneration and the damage, and to evaluate the ocular irritation of the subject.

what are the dangers of reactive oxygen free radicals (ros) to the skin?

reactive oxygen species (ros) are chemically reactive chemicals containing oxygen. examples includesuperoxide，hyperoxide， hydroxy radical, singlet oxygen, and α -oxygen. when the skin receives external factors of stimulation, including radiation, cigarette smoke, air pollution, etc., reactive oxygen species production increases significantly.

the overproduction of free radicals is the main factor causing skin aging.

free radicals will attack the skin cell membranes, destroy the normal structure of the skin cells, making them lose their original function, which can also lead to skin aging. in addition, reactive oxygen free radicals are able to activate melanocyte proliferation and tyrosinase-related gene expression through multiple signaling pathways to improve melanin synthesis. thus resulting in melanin deposition, dark skin, color spots and other problems.

the role of mitochondria in skin aging?

skin aging is the result of the loss of cell function, and external factors can further accelerate the loss of cell function. mitochondria play an important role in skin function, and mitochondrial damage has been found not only to accumulate in skin cells with age, but also in response to sunlight and pollution. increasing evidence suggests that mitochondrial dysfunction and oxidative stress are key features of all aging tissues, including the skin. this is directly related to the skin aging phenotype: wrinkle formation, hair whitening and shedding, uneven pigmentation, and slow wound healing. loss of barrier function during skin aging increases susceptibility to infection and affects wound healing. therefore, understanding the relevant mechanisms is important for both clinical research and the development of anti-aging skin care products.

the relationship between t rpv1 and soothing efficacy?

the surface of keratinocytes will express some receptors that can feel temperature and pain, such as trp, the most typical trp receptors including capsaicin receptor (trpv1), external or endogenous stimuli will activate trpv1, thus causing ca2 influx, resulting in increased skin permeability, tingling and burning sensation. meanwhile, trpv1 also promotes the release of neurogenic inflammatory factors, including substance p, which can further induce the molecules of inflammatory factors, leading to local telangiectasia.

therefore, we can evaluate the soothing efficacy of the objects to be tested by detecting the changes in trpv1 expression amount, calcium ion content, and inflammatory factors.

our advantage

authoritative qualification

noa has been approved by certification and accreditation administration of the people’s republic of china（cnca）(cnca-r-2002-051), and has obtained qualification of china inspection body and laboratory mandatory approval (cma),and has passed the multiple approvals of china national accreditation service for conformity assessment (cnas), international accreditation service (ias), united kingdom accreditation service (ukas), joint accreditation system of australia and new zealand (jas-anz). noa has been approved by state administration for market regulation of china, and has been recognized as inspection and testing institution of china's special equipment, as well as the qualification of china's national equipment supervision and engineering supervision. noa-dci is the notified body of the ce directive of the european commission. noa has been recognized by the international electrotechnical commission (iecq) by obtaining electronic component quality assessment system. it is also a national inspection and assessment notified body of import and export commodity in china.noa is a high-tech enterprise in shanghai.

improve performance, realize asset value appreciation, and service throughout the entire value chain

from pre-design to post-operation, noa has the ability to guarantee the whole life cycle of the business. noa, as an independent third-party inspection company, has a large number of domestic and international standards and specifications proficient in design, welding, non-destructive testing, painting, packaging and other fields the experienced team of professional engineers and inspection experts, with more than ten years of experience in the domestic market, is familiar with all aspects of the domestic industrial equipment supply chain, and can provide you with technical support services for the full life cycle of technical services in a timely manner, combining various products. inspection, certification, testing, consulting, and auditing services can provide you with one-stop all-round comprehensive services.

quality, efficiency and service

noa has formed a mature and solid operation system in the development of more than 20 years. we let technical experts who are familiar with market regulations and testing standards and have professional industry experience to carry out inspection, evaluation and design review work. while meeting the requirements of domestic and international standards, we ensure that customers can obtain satisfactory service results in the first time with accurate time-sensitive management methods, and ensure that customers can seize the opportunity in the market competition.

service area

noa inspection services currently cover europe, australia, russia, some middle east regions and most regions in china. noa can ensure the consistency and continuity of customer service in different regions, and eliminate the impact of unfamiliar environments on customer quality. the guarantees and the impact of project implementation enable customers to participate in different markets across the country or around the world with flawless quality.

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